Journal: Cancer research
Article Title: Multimodal molecular imaging detects early responses to immune checkpoint blockade
doi: 10.1158/0008-5472.CAN-20-3182
Figure Lengend Snippet: (A) Flow cytometry analysis of tumor infiltrating lymphocytes and tumor draining lymph node cells. Tumor bearing mice were treated with each Ab on day 9 and 12. Tumors and lymph nodes were harvested on day 13. The figure on each plot shows the percentage of CD3+CD4+ T cells or CD3+CD8+ T cells. (B and C) Immunofluorescence staining of CD3 and CD8 in MC38 tumor (B) or B16-F10 tumor (C) (scale bar = 100 μm). Tumor bearing mice were treated with each Ab on day 9 and 12. Tumors were harvested on day 13. Number of cells were manually counted in randomly selected 5 fields per tumor section (MC38; n = 4 per group, B16-F10; n = 5 per group). Data are shown as mean ± SE. Statistical significance between groups was determined by Student’s t test.
Article Snippet: To analyze tumor infiltrating lymphocytes, single-cell suspensions were prepared from solid tumors by digestion with a mixture of 0.1 % collagenase, 0.01 % DNase, and 2.5 U/ml hyaluronidase for 3 h at 37 deg C. FITC-conjugated mAbs against CD4 (RM4-5); PE-conjugated mAbs against CD3; Cy-chrome-conjugated mAbs against CD8 (53-6.7) were purchased from BD Biosciences.
Techniques: Flow Cytometry, Immunofluorescence, Staining